FOR RESEARCH USE ONLY. A phosphate buffer solution is a handy buffer to have around, especially for biological applications. That is because the buffering capacity of a phosphate buffer is pH 7.21 ± 1 within which most of the proteins and enzymes are more stable. This product is a tablet for preparing Phosphate Buffered Salts (PBS), which are commonly used for ELISA, Western Blotting and immunohistochemical staining. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. ©2018 Takara Bio Inc. All Rights Reserved. (0)77.565.6999FOR RESEARCH USE ONLY. Choose the buffer species you want to use, and enter parameters for volume, pH, and concentration of buffer species. *You must be logged in to a Business Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Takara Bio USA, Inc.United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33. Mix appropriate volumes of stock and add an equal volume of distilled water to make a final 0.1 M Sørensen’s phosphate buffer solution (Sørensen, 1909; Gomori, 1955). Our mission is to develop high-quality innovative tools and services to accelerate discovery. SØRENSEN’S PHOSPHATE BUFFER; PH 5.8–8.0, PK A = 7.20. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Application procedure: Dissolve 1 tablet in 200 mL water to obtain a 137 mM NaCl, 2.7 mM KCl and 10 mM phosphate buffer solution (pH 7.4 at 25 °C) 0.1 M sodium phosphate buffer (from 1 M stocks) at 25°C. Phosphate buffered saline (a.k.a PBS or PBS buffer) is one of the most used buffers since it is isotonic to most of the cells. Takara Bio is proud to be on the front line in the fight to defeat the novel coronavirus by enabling innovative vaccine development. We have placed reagents required for preparation in a table. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED). desired pH. phosphate buffer. The buffer can easily be prepared by dissolving the tablet in H2O. Phosphate Buffer Preparation Table – 0.2M solution. 2.6–7.6. This product is a tablet for preparing Phosphate Buffered Saline (PBS), which is commonly used for ELISA, Western Blotting and immunohistochemical staining. Phosphate buffer. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion. Mixing 1 M NaH2PO4 (monobasic) and 1 M Na2HPO4 (dibasic) stock solutions in the volumes designated in the table below results in 1 L of 1 M sodium phosphate buffer of the (0)1.3904.6880 • Japan: +81. The buffer can easily be prepared by dissolving the tablet in H2O. Then, include the option to modify the ionic strength by addition of neutral salt. Finally, enter the temperature at which you'll use the buffer, and the temperature at which you'll make it up (these are often not the same). Additional product, intellectual property, and restricted use information is available at takarabio.com. An account with takarabio.com entitles you to extra features such as: • Creating and saving shopping carts • Keeping a list of your products of interest • Saving all of your favorite pages on the site* • Accessing restricted content. This product is a tablet for preparing Phosphate Buffered Saline (PBS), which is commonly used for ELISA, Western Blotting and immunohistochemical staining. To prepare 1 L of 0.1 M sodium phosphate buffer of the desired pH, the following mixtures should be diluted to 1 L (final Read our, SmartChip Real-Time PCR System, chips, and reagents, RNA extraction and analysis for real-time qPCR, Primary antibodies and ELISAs by research area, SmartChip Real-Time PCR System introduction, Guest webinar: extraction-free SARS-CoV-2 detection, Phosphoprotein and glycoprotein purification, Exploring OEM and custom enzyme partnerships, Outsourcing stem cell-based disease model development, Gene and cell therapy manufacturing services, Characterizing the viral genome and host response, Immunizing mice and optimizing vaccine targets, Gene editing for cancer therapy/drug discovery, iDimerize inducible protein interaction systems, Custom business friendly and automation-ready solutions, SMARTer RACE 5'/3' Kit—advances in SMARTer PCR cDNA synthesis, DSS Takara Bio India Pvt.
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